The chloro-phosphonium coupling reagent PyCloP was developed as an alternative to the BOP reagent, avoiding the release of the toxic HMPA in the activation step.
Synonym: Chlorotripyrrolidinophosphonium hexafluorophosphate
C12H24ClN3P · PF6
White to off-white crystalline powder
Store in a cool and dry place (2-8oC)
- PyCloP often gives better results than other phosphonium-HOBt reagents, for the coupling of N-methylated amino acids.
- PyCloP, as other phosphonium salts, cannot react with free amine function and therefore does not terminate the peptide growing as the aminium salts do, through the formation of guanidine derivatives.
- Coupling N-Methylated Amino Acids Using PyBroP and PyCloP Halogenophosphonium Salts: Mechanism and Fields of Application
J. Coste, E. Frerot, P. Jouin J. Org. Chem., 1994, 59 (9), pp 2437–2446.