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EDC·HCl, usually known as water soluble carbodiimide, is a versatile modern coupling agent.

CAS: 25952-53-8

Synonym: 1-Ethyl-3-(3-dimethyllaminopropyl)carbodiimide hydrochloride




Molecular Formula


Molecular Weight

191.7 [gr/mol]


White to off-white crystalline powder

Storage Conditions

Store in a cool place


  • It is an easily handled solid with high solubility in water (>200gl) and organic solvents such as dichloromethane, tetrahydrofuran and dimethylformamide.
  • EDC·HCl replaced N,N-Dicyclohexylcarbodiimide (DCC) in solution-phase peptide synthesis because its urea is highly soluble in aqueous solutions, and so is easily removed by an aqueous work-up.
  • As well as being used for the synthesis of amides, EDC·HCl is also used as a coupling agent in the preparation of esters from carboxylic acids using dimethylaminopyridine as the catalyst.
  • Combinations of EDC·HCl and potassium salts of oximes are new and unique coupling cocktails that could be used in particular for the formation of hindered peptides such as Aib-enkephaline pentapeptide.


  • DC.HCl and Potassium Salts of Oxyma and Oxyma-B as Superior Coupling Cocktails for Peptide Synthesis
    Y. E. Jad, S. N. Khattab, B. G. de la Torre, T. Govender, H. G. Kruger, A. El-Faham, and F. Albericio Eur. J. Org. Chem., 2015, 14, 3116–3120.
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  • Evaluation of alternative solvents in common amide coupling reactions: replacement of dichloromethane and N,N-dimethylformamide
    D. S. MacMillan, J. Murray, H. F. Sneddon, C. Jamieson, and A. J. B. Watson Green Chem., 2013, 15, 596–600.
  • Thermal Stability Assessment of Peptide Coupling Reagents Commonly Used in Pharmaceutical Manufacturing
    Jeffrey B. Sperry*, Christopher J. Minteer, JingYa Tao, Rebecca Johnson, Remzi Duzguner, Michael Hawksworth, Samantha Oke, Paul F. Richardson, Richard Barnhart, David R. Bill, Robert A. Giusto, and John D. Weaver III
    Org. Process Res. Dev. 2018, 22, 9, 1262–1275

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