HBTU is coupling reagent for peptide synthesis.

CAS: 94790-37-1

Synonym: N-[(1H-benzotriazol-1-yl)(dimethylamino)methylene]-N-methylmethanaminium hexaflourophosphate N-oxide




Molecular Formula


Molecular Weight

379.30 [gr/mol]


White to off-white crystalline powder

Storage Conditions

Store in a cool and dry place (20oC)


  • Recent studies show that crystal as well as solution structure of this coupling agent is a guanidinium N-oxide and not an uronium compound.
  • HBTU is attributed to various advantages, such as -very low racemization, simple reaction conditions, very short reaction time, and high yields.


  • HBTU mediated 1-hydroxybenzotriazole (HOBt) conjugate addition: synthesis and stereochemical analysis of β-benzotraizoleN-oxide substituted γ-amino acids and hybrid peptides
    S. M. Mali, M. G. Kumar, M. M. Katariya and   H. N. Gopi Org. Biomol. Chem., 2014, 12, 8462-8472.
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  • Structural studies of reagents for peptide bond formation:Crystal and molecular structures of HBTU and HATU
    I. Abdelmoty, F. Albericio b, L. A. Carpino, B. M. Foxman, and S. A. Kates Letters in Peptide Science, 1994, 1, 57-67.
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  • Peptide Coupling Reagents, More than a Letter Soup
    A. El-Faham and F. Albericio. Chem. Rev., 2011, 111, 6557-6602.

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