DEPBT is an effective coupling reagent for synthesis of linear and cyclic peptides by both solution and solid-phase peptide synthesis.

CAS: 165534-43-0

Synonym: 3-(Diethoxyphosphoryloxy)-1,2,3-benzotriazin-4(3H)-one




Molecular Formula


Molecular Weight

299.2 [gr/mol]


White to off-white crystalline powder

Storage Conditions

Store in a cool and dry place (20oC)


  • DEPBT mediates amide bond formation with remarkable resistance to racemization.
  • When DEPBT is used as a coupling reagent, it is not necessary to protect the hydroxy group of the amino component (such as tyrosine, serine, and threonine) and the imidazole group in the case of histidine.
  • DEPBT has been shown to be highly effective for cyclolactamization of peptides.


  • DEPBT as an efficient coupling reagent for amide bond formation with remarkable resistance to racemization
    Y. Ye, H. Li, and X. Jiang Peptide Sience, 2005, 80, 2-3, 172–178.
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  • DEPBT as Coupling Reagent To Avoid Racemization in a Solution-Phase Synthesis of a Kyotorphin Derivative
    V. G. Ramu, E.  Bardaji, and M. Heras  Synthesis 46(11):1481.
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